Rapid, reproducible enzyme immunoassay for tobramycin.
نویسندگان
چکیده
An enzyme immunoassay for tobramycin utilizing glucose 6-phosphate dehydrogenase was compared with the radioimmunoassay. The enzyme immunoassay for tobramycin was accurate, specific, and easily performed. It offers an alternative method for assaying aminoglycosides and could be used in institutions that use the enzyme immunoassay to assay other drugs.
منابع مشابه
Comparison of enzyme-multiplied immunoassay technique with fluorescence polarization immunoassay for determination of gentamicin and tobramycin levels in serum.
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An enzyme immunoassay for gentamicin utilizing glucose-6-phosphate dehydrogenase was compared to the radioimmunoassay. The enzyme immunoassay for gentamicin was accurate, specific, and easily performed. It offers an ulternative method to assay aminoglycosides and could be used in institutions using enzyme immunoassay to assay other drugs.
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Four assay procedures for tobramycin in serum--enzyme immunoassay (I), substrate-labeled fluorescent immunoassay (II), radioimmunoassay (III), and bioassay (IV)--were compared and evaluated by replicate and analytical recovery studies. I and II were about 50% more precise than III and IV. II was substantially more nearly accurate than the other methods and also gave the best reproducibility (co...
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 13 1 شماره
صفحات -
تاریخ انتشار 1981